摘要 :
Grafting copolymerization of styrene and acrylic acid onto polypropylene fabric had been studied by using a pre-irradiation technique. The polyproylene fabric samples were irradiated by γ-ray and electron beam under the condition...
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Grafting copolymerization of styrene and acrylic acid onto polypropylene fabric had been studied by using a pre-irradiation technique. The polyproylene fabric samples were irradiated by γ-ray and electron beam under the conditions of air and nitrogen gas, respectively. The effect of absorbed dose, monomer concentration in solvent, reaction temperature and reaction time on the degree of grafting were determined.
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摘要 :
AIM: To localize the active site of ribosome inactivation of trichosanthin (Tri), a Chinese herb protein. METHODS: Hydroxylamine was used to specifically cleave the unique Asn-Gly peptide bond of Tri. Preparative SDS-polyacrylamid...
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AIM: To localize the active site of ribosome inactivation of trichosanthin (Tri), a Chinese herb protein. METHODS: Hydroxylamine was used to specifically cleave the unique Asn-Gly peptide bond of Tri. Preparative SDS-polyacrylamide gel electro- phoresis was applied to get 2 cleaved fragments, HATfl and HATf2. Western blotting was used to determine the different epitopes of Tri and screen the antibodies. A cell-free system, rabbit reticulocyte lysate, was introduced to quantitate the inhibitory activity of Tri and its fragments on protein biosynthesis. RESULTS: HATf1 and HATf2 were separated with the purity of 96.9/100 and 80.5/100 respectively. HATf1, like intact Tri, retained the inhibitory activity on protein biosynthesis.
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